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Flow cytometry with gold nanoparticlesand their clusters as scattering contrast agents: FDTD simulation of light-cell interaction

机译:金纳米颗粒及其簇作为散射造影剂的流式细胞术:光细胞相互作用的FDTD模拟

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摘要

The formulation of the Finite-Difference Time-Domain (FDTD) approach is presented in the framework of its potential applications to in vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets.
机译:在其基于光散射的体内流式细胞术的潜在应用框架中,介绍了时差有限域(FDTD)方法的制定。考虑集中在比较单个生物细胞在受控的折射率匹配条件下与金纳米颗粒标记的细胞的光散射。还分析了包括相差(OPCM)显微镜作为流式细胞术的预期形式的光学原理图。用于流式细胞术模拟的FDTD方法的验证可能会为基于纳米靶标的散射效应的先进细胞术技术的开发开辟新的途径。

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